Fig. 8. LY294002 treatment attenuated chemotaxis defects of sodC^– cells. (A) Cells expressing the PtdIns(3,4,5)P₃ marker GFP-PHcrac were pulsed for 4 hours with 50 nM cAMP, and either left in DB buffer or treated with 15 µM or 50 µM LY294002 (LY) for 20 minutes. GFP-PHcrac aberrantly localized at the plasma membrane of sodC^– cells but not of wild type. Membrane localization of GFP-PHcrac in sodC^– cells largely disappeared after LY treatment. GFP signals at the membrane of sodC^– cells were reminiscent of fine filopodial extensions, which also disappeared after LY treatment. (B) Wild-type and sodC^– cells were pulsed for 4 hours with 50 nM cAMP, and treated with and without 15 µM LY294002 (LY) for 20 minutes. Cells were then challenged with micropipettes filled with 10 µM cAMP for 20 minutes. Twenty stacks of cell tracing images 1 minute apart, are shown with a 100 µm scale bar. (C) Roundness values of the analyzed cells are shown.