Fig. 4. Subcellular localization of the DPH4 protein. NIH-3T3 cells transfected with FLAG-tagged DPH4 (all panels) and myc-tagged mouse DPH1 (OVCA1, upper panels) are shown. Cells were subsequently stained with anti-FLAG antibodies to detect DPH4 (green), anti-myc to detect mouse DPH1 (red, upper panels) or with phalloidin to detect microfilaments (middle and lower panels). Right-hand panels are merged images.