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Figure 2


Fig. 2. Accumulation of GFP-tagged MSH2, MSH3 and MSH6 at the laser-irradiated site. (A) Accumulation kinetics of GFP-tagged MSH2, MSH3 and MSH6 at laser-irradiated site in HeLa cells. (B) GFP-MSH3 and MSH6 but not mDR-MSH2 accumulate at laser-irradiated site in HCT116. (C) mDR-MSH2 cotransfected with GFP-MSH3 or with GFP-MSH6 (D) accumulates at laser-irradiated site in HCT116 cells. (E) Western blot analysis of MSH2 in HeLa and LoVo cells (left). Accumulation of GFP-MSH3 and GFP-MSH6 at laser-irradiated site in LoVo cells (right). (F) Accumulation of GFP-MSH3 and GFP-MSH6 at laser-irradiated site after suppression of MSH2 expression in HeLa cells. (G) Influence of suppression of either MSXH3 or MSH6 on the accumulation of GFP-MSH2 at laser-irradiated site in HeLa cells. (H) Influence of suppression of both MSXH3 and MSH6 on the accumulation of GFP-MSH2 at laser-irradiated site in HeLa cells. More than 10 cells are analyzed for each condition and representative data are shown.