Fig. 9. ErbB2 is delivered to late endosomes and lysosomes after GA treatment. After transient expression of GFP-Rab7 (A only), SK-BR-3 cells were treated with GA for 5 hours (together with 0.1 mg/ml leupeptin, D only), fixed and permeabilized. Left panels: ErbB2 was detected with polyclonal (A,B) or monoclonal (C,D) antibodies and appropriate secondary antibodies. Middle panels: (A) Rab7 (GFP fluorescence); (B) endogenous CD63; (C,D) endogenous LAMP1. Right panels: merged images. Epifluorescence images are shown. Scale bar: 5 µm.