Fig. 1. CAV-1 bodies/cortical granules deliver chondroitin proteoglycans to extracellular space after fertilization. (A,B) The gonads of transgenic animals expressing CAV-1::GFP were stained with an anti-chondroitin antibody (A) or WGA-TRITC (B). CAV-1 bodies were stained with the anti-chondroitin antibody and WGA-TRITC, indicating that these structures contain chondroitin proteoglycans and mucin-like glycoproteins. (C) The gonads of N2 wild-type worms were stained with anti-chondroitin antibody and WGA-TRITC. The right panels are 4x enlargements of the boxed area indicated in merged images. Scale bars: 10 µm. (D) Stylized drawing of one gonad arm connected to the spermatheca and the uterus. Oocytes and embryos are numbered according to their positions from the spermatheca. Schematic localization of CAV-1::GFP is indicated in green. Chondroitin proteoglycans and mucin-like glycoproteins are indicated in orange. Developmental events are assembly-line-like processes and take place at indicated positions of the germline.