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Figure 6


Fig. 6. RAB-11.1 and EMB-27 are required for proper eggshell formation after fertilization. The permeability of embryos to FM4-64 was examined in mock (A), emb-27(RNAi) (B) and rab-11.1(RNAi) (C) animals expressing CAV-1::GFP. A newly fertilized wild-type embryo is permeable to FM4-64 (+1 embryo in A, an arrow indicates the PM stained with FM4-64) but blastomeres are not (+2 and +3 embryos in A). The emb-27(RNAi) and rab-11.1(RNAi) embryos are permeable to FM4-64. Nomarski image is on the right in each pair. Scale bar: 10 µm.