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Figure 1


Fig. 1. Processing of pNedd8 by DEN1. (A) DEN1 processes His-pNedd8GG in a concentration-dependent manner (0.05-0.3 µg, lanes 2-5). The processed product is equivalent to the purified His-mNedd8 in mobility (lane 6, indicated by arrow). However, His-pNedd8AA (0.3 µg) was not processed by DEN1 (lane 7). (B) In a similar set of experiments, DEN1CA fails to process His-pNedd8GG. Asterisks in A,B indicate a non-specific signal present in the preparation of His-pNedd8GG.