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Figure 2


Fig. 2. The cyclin E-interaction domain within MCM5. (A) HA-tagged MCM5 cyclin E-interaction fragments. The indicated HA-tagged MCM5 fragments were radiolabeled with [35S]methionine in an in vitro transcription-translation system and subjected to pulldown analysis as in Fig. 1B with GST or GST–cyclin E. Right: an autoradiograph is shown. Left: a Coomassie-stained gel of 25% of GST and GST cyclin E. (B) GFP-tagged MCM5 cyclin E-interaction fragments. The indicated GFP-tagged fragments were radiolabeled and analyzed by GST pulldown analysis as in A. Right: autoradiograph of pulldown. Left: Coomassie-stained gel of 25% of GST and GST proteins.