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Figure 4


Fig. 4. Localization of MCM5 on centrosomes. (A) Endogenous MCM5. Asynchronous CHO-K1 or HeLa S3 cells were methanol-fixed and stained with antibody to {gamma}-tubulin and MCM5, as indicated. Arrowheads mark the centrosome. Inset: enlarged image of the centrosomal area. (B) Transfection of HA-tagged MCM5. CHO-K1 cells were transiently transfected with the indicated MCM5 constructs as described in the Materials and Methods. Cells were methanol-fixed and stained with antibodies to {gamma}-tubulin and HA, as indicated. Arrowheads mark the centrosome. Inset: enlarged image of the centrosomal area. (C) Transfection of GFP-tagged MCM5 fragments. The indicated constructs were expressed in CHO-K1 cells and stained with antibody to {gamma}-tubulin. GFP was visualized by excitation of the expressed GFP tag. Arrowheads mark the centrosome. Inset: enlarged image of the centrosomal area. Scale bars: 5 µm.