Fig. 7. Characterisation of the metaphase-like cells in Stra8^–/– testes. (A-H) Immunodetection of SYCP3 (green signal), H2AX (red signal) and DAPI nuclear counterstain (blue) on histological sections. (I-L) Double immunolabelling for detection of SYCP3 (green signal), H2AX (red signal) and DAPI nuclear counterstain (blue) on nuclear spreads. (M-P) Giemsa-stained metaphase spreads. Note that panels M,N correspond to WT meiotic and mitotic (i.e. spermatogonial) metaphases, respectively. L/Z, leptotene/zygotene spermatocytes; M, cells in meiotic metaphase (WT); M^*, metaphase-like (Stra8^–/–) cells; XII, stage XII of the seminiferous-epithelium cycle. Scale bar: 30 m (A-H); 10 m (I-P).