Fig. 3. The RRR ER-retention/retrieval signal reduces surface expression of the CD8 chimeras. COS-7 cells were transfected with plasmids expressing Myc-CD8, Myc-CD8-KKTN, or with a series of constructs with the C-terminus of CD8 linked to 2L or to the various segments of 1L of Na_v1.8. Non-permeabilized and permeabilized immunofluorescence labeling were carried out with antibodies against Myc. The images are representative of at least three independent experiments. Scale bar: 10 µm.