Fig. 1. Zinc treatment induces disassembly of polysomes. (A) P19 neurons were treated with 100 M zinc for the indicated times. The cell lysate was loaded onto a 15-45% linear sucrose gradient. Gradient fractions were collected from the top, and equal volume samples were separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and analysed for ribosomal proteins distribution. (B) RNA was isolated from the gradient fractions and separated on 1% agarose gel. (C) Statistical analysis of the ribosomal proteins content in fractions 4-6. Bars represent mean ± s.e.m., n 3. ^*P<0.05 using Student's t-test. (D) Statistical analysis of the ribosomal proteins content in fractions 7-10. Bars represent mean ± s.e.m., n 3. ^*P<0.05, Student's t-test.