Fig. 4. Zinc application induces the aggregation of Dcp1a protein in dense sucrose gradient fractions. P19 neurons were treated with 100 M zinc for the indicated times. Cell lysate was loaded on 15-45% linear sucrose gradient and gradient fractions were collected from the top and analysed for Dcp1a protein distribution. (A) SDS-PAGE of equal volume samples of the gradient fractions. (B) Statistical analysis of Dcp1a protein levels in gradient fractions 6-10 of cells treated with zinc for 2 hours, compared with control cells. Results were normalized to 2% of the cell lysate Bars represent mean ± s.e.m., n 3. ^*P<0.05, Student's t-test.