Fig. 5. Dcp1a aggregation in response to zinc treatment is RNA dependent. P19 neurons were treated with 100 M zinc and harvested. Cell lysates were incubated with 1 g/l RNase A for 30 minutes at room temperature, prior to sucrose gradient centrifugation. Gradient fractions were collected and subjected to western blot analysis. Western blot analysis (A) using Dcp1a antibody and (B) using HuD antibody, as a control.