Fig. 5. LIM2-LIM3 displaces full-length GFP-paxillin from focal adhesions. Cells microinjected with GST-LIM2 (A,B) and GST-LIM2-LIM3 (C,D) proteins were stained with antibodies against GST (A,C) and vinculin (B,D). Only GST-LIM2-LIM3 (C,D) localizes to focal adhesions. Bar 10 µm. Cells expressing GFP-paxillin (E,G) were microinjected with either GST-LIM2-LIM3 (F) or GST only (H). By 45 minutes after injection of GST-LIM2-LIM3, paxillin is displaced from existing focal adhesions (F, arrows) and does not appear in new adhesion sites. Microinjection of just GST changed neither the intensity level of paxillin in old adhesions (H, arrows) nor the ability of paxillin to incorporate into new adhesion sites (H, arrowheads). Bar, 10 µm.