Fig. 5. feSEM of nuclear envelope spreads of oocytes expressing lamin A. FLAG-tagged lamin A was expressed by nuclear injection of a small amount (0.38 ng) of plasmid DNA. (A-C) Small filamentous aggregates are marked by arrows in A and C, and immuno-gold labels against FLAG are indicated by gold spots in B. (D,E) NPC baskets are indicated by arrowheads in D, and a multilayer filament region is indicated by large arrows in D and E. Image E is a red-green 3D stereo pair of an area similar to that in D and requires red-green glasses.