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Figure 4


Fig. 4. Intercellular junctions differ between myofibroblasts and fibroblasts. (A) Expression of myofibroblast marker {alpha}-SMA, gap junction marker Cx43 and adherens junction markers pan-cadherin and β-catenin was assessed together with loading control vimentin, by western blotting. The cytoskeletal fractions are compared after 1-7 days culture between control fibroblasts (lanes labelled C) and fibroblasts treated with TGFβ1 to induce myofibroblast differentiation (lanes labelled T). (B) Band signal strength in western blots was analysed by optical densitometry and related to loading control vimentin (n=3). After 4 days of growth, Triton-X-100-extracted myofibroblasts (C) and fibroblasts (D) were immunostained for β-catenin (green), Cx43 (red) and {alpha}-SMA (blue). Scale bars, 25 µm.