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Figure 3


Fig. 3. Effect of TGFβ signals on Snail expression in MESECs. (A) Levels of expression of Snail in MESECs cultured in the absence (–) or presence of TGFβ2 (Tβ) or TβR-I inhibitor (inhib) as analyzed by quantitative real-time RT-PCR. Error bars indicate s.d. (B) Protein levels of Snail (top) and {alpha}-tubulin (bottom) were examined by immunoblotting of total lysates of the HUVECs cultured in the absence or presence of TGFβ2 or TβR-I inhibitor.