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Figure 5


Fig. 5. Effect of Snail on expression of endothelial and mural markers in MESECs. (A-D) Levels of expression of claudin 5 (A), SMA (B), SM22{alpha} (C) and calponin (D) in MESECs derived from Tc-Empty or Tc-Snail ESCs cultured in the absence (–) or presence (+) of Tc were analyzed by quantitative real-time RT-PCR. Error bars indicate s.d. (E) Protein levels of claudin 5 (top), SMA (middle) and {alpha}-tubulin (bottom) were examined by immunoblotting of total lysates of the MESECs described in A-D.