Fig. 2. TM9SF4 gene map. (A) The TM9SF4 gene produces one transcript of 2.6 kb which contains one coding sequence (coloured in grey). The insertion point for {lacW}CG7364^k07245 is 94 bp upstream of the ATG translation start. One 1.4 kb deletion (TM9SF4¹) was recovered encompassing the transcription start site and the N-terminal part of the corresponding protein. (B) The deletion creates a null allele as visualised by northern analysis of TM9SF4 transcripts in control w¹¹¹⁸ (lane 1) compared with mutant TM9SF4¹ (lane 2) flies. (C) Developmental northern blot. Lane 1, embryos; lane 2, third instar larvae; lane 3, pupae; lane 4, adult. (D) TM9SF4 transcripts were quantified by real-time PCR from total RNAs extracted from either the whole third instar larvae (L3), the gut (Gut), the fat body (FB) or the larval circulating plasmatocytes (He). Results are mean ± s.d.