Fig. 3. Survival rate of infected Drosophila flies. 5- to 7-day-old males, previously raised at 25°C, were infected with indicated bacteria, either by septic injury onto the thorax with a thin needle previously dipped into the indicated bacterial solution (A,C-G) or by oral ingestion (B). Survival rate was followed at 25°C except in the case of S. aureus (20°C) as indicated. (A-F) Survival of TM9SF4 mutant flies and TM9SF4/Df(2L)b82a2 compared with control w¹¹¹⁸ (w), Rev45 flies or dTAK1² (TAK1) mutant flies affected in the Imd pathway. (G) The number of colony forming units (CFUs), plotted in logarithmic scale, was calculated from bacteria isolated from infected flies.