Fig. 1. Analysis of the putative mouse neuroserpin promoter. Regions of the putative mouse neuroserpin promoter were subcloned into pGL3-Basic vector, upstream of the firefly luciferase gene. Mv1Lu (mink lung epithelial) cells were transiently transfected with the pRL-TK control vector and pGL3-Basic vector containing the different regions of the mouse neuroserpin promoter. Firefly and Renilla luciferase activities were measured as described in the Materials and Methods. After normalization to Renilla luciferase activity, values were expressed as a percentage of the activity of construct A (valued as 100%) (mean ± s.d.; n=8). Asterisks indicates a significant difference from the A construct: ^*P<0.05, ^**P<0.01, ^***P<0.001; hash marks indicate a significant difference from the E construct: ^#P<0.05, ^##P<0.01; Kruskall-Wallis test followed by a Mann-Whitney post-hoc test.