Fig. 3. Regulation of neuroserpin expression in vitro. (A) mRNA levels of neuroserpin (NS), tPA, PAI-1 and protease nexin 1 (PN1) in cultured neurons (N; n=3) and astrocytes (A; n=3). (B) Evaluation of the mRNA levels for neuroserpin in cultured neurons (n=5) and astrocytes (n=3) treated over 24 hours with either recombinant BMP2, BMP4, BMP7 or AMH at a concentration of 1 or 10 ng/ml. Relative levels of mRNA expression were measured by quantitative PCR as described in the Materials and Methods. C, control. Results were computed by calculating both the 2^–Ct (white bars) and the 2^–Ct (grey bars). Bars represent mean values ± s.d. (2^–Ct:^*P<0.05, ^**P<0.01; 2^–Ct: ^#P<0.05, ^##P<0.01; Kruskall-Wallis test followed by Mann-Whitney post-hoc test).