Fig. 8. Neuroprotective effect of AMH against NMDA-induced excitotoxicity. (A) Pure cultures of neurons (14 DIV) were exposed for 24 hours to NMDA (12.5 µM) without (black bars) or with (white bars) increasing concentrations of AMH (1 or 10 ng/ml) and neuronal death was measured as described in the Materials and Methods (n=4). Asterisks indicate a significant difference from NMDA alone by one way Kruskall-Wallis test followed by Mann-Whitney post-hoc test (^*P<0.05). (B) Effects of intra-striatal injection of recombinant AMH (0.45 pg) on the extent of neuronal death induced by the striatal administration of NMDA (10 nmol) in mice (n=9). Bars represent mean values ± s.e.m. ^**P<0.01; Mann-Whitney test. Diagram on the right illustrates on a brain section the typical lesion size obtained by the striatal injection of NMDA alone versus NMDA + AMH.