Fig. 4. The level of JIL-1 expression does not, but JIL-1 binding to chromatin is severely decreased in ATAC mutants. (A) The level of JIL-1 mRNA in ATAC and JIL-1 mutants determined by real-time RT-PCR. (B) Immunoblot of total protein extracts of dAda2a^d189- and dAda2b^d842-null mutant and wild-type third-instar larvae developed using JIL-1-specific antibody. (C) Immunostaining of salivary glands of ATAC- and JIL-1-null mutants using JIL-1-specific antibody. (D) Immunostaining of polytene chromosomal spreads (top) and `smush' preparations (bottom) of wild-type and dAda2a^d189, dGcn5^E333st and JIL-1^z2 third-instar larvae using JIL-1-specific antibody. Chromosomes of males are shown. The JIL-1 mutant shown is null homozygote.