Fig. 2. Wdb exerts its effects on FOXO in the eye via its PP2A-B' regulatory activity. The reduced eye phenotype produced by overexpression of foxo^GS9928 under GMR-GAL4 control (F) is enhanced by co-overexpressing wdb^GS9548 (G). When wdb^GS9548 is expressed alone (B), the eye is reduced and disorganised relative to wild-type controls (A). Flies heterozygous for the recessive lethal mts allele mts⁰²⁴⁹⁶ show no obvious eye defects (C). This allele also appears to have little, if any, effect on the FOXO phenotype (H). Although mts⁰²⁴⁹⁶ does not noticeably modify the phenotype produced by overexpressing wdb^GS9548 (D), it does suppress the reduced eye phenotype caused by co-overexpression of wdb^GS9548 and foxo^GS9928 (I). Overexpression of a dominant-negative form of Mts with GMR-GAL4 induces overgrowth (bulged eye in E) and completely suppresses the FOXO phenotype (J).