Fig. 5. Wdb regulates lipid droplet size in nurse cells by inhibiting accumulation of cytoplasmic pAkt1. Clones mutant for wdb^IP (A-D,I-K,O-V), for wdb^dw (E-H), and for both wdb^IP and Akt1¹ (L-N,W-Z) were generated in combination with a normal GFP-labelled third chromosome, and stained with phalloidin to detect the actin cytoskeleton (C,G), with an antibody against activated pAkt1 (B,F), with Nile Red to detect neutral lipid (J,M,P), with Hoechst 33258 to detect nuclei (Q,U,Y) and with an anti-LSD2 antibody (T,X). Mutant clones were identified by lack of GFP expression (A,E,I,L,O,S,W and merges in D,H,K,N,R,V,Z). wdb mutant clones contain cells with normal nuclei, elevated levels of cytoplasmic pAkt1 and large lipid droplets (arrows in J,P). LSD2 protein levels are also increased (T). The lipid droplet phenotype is completely suppressed by the Akt1¹ hypomorphic allele (M), but the LSD2 phenotype is only partially suppressed (X). Most egg chambers are at stage 10, but E is at stage 9 and O is at stage 11 of oogenesis. Scale bars: 50 µm.