Fig. 7. Wdb regulates levels of cytoplasmic pAkt1 and LSD2 in ovarian follicle cells. Clones mutant for wdb^IP (A-L), for wdb^IP and Akt1¹ (M-P) and PTEN¹ (Q-B') were generated in combination with a normal GFP-labelled third chromosome, and stained with phalloidin to detect the actin cytoskeleton (C,S), with an antibody against activated pAkt1 (B,F,R,V), with anti-LSD2 (J,N,Z; image in Z is at higher gain to show normal low levels of LSD2 staining) and with Hoechst 33258 to detect nuclei (G,K,O,W,A'). Although pAkt1 levels and LSD2 expression are upregulated in all wdb mutant clones (B,F), only a minority of PTEN mutant clones exhibit these phenotypes (compare R with V,Z). Note that the LSD2 phenotype in wdb mutant clones (J) is partially suppressed by the Akt1¹ mutation (N). When this Akt1 mutation is combined with a wdb mutation, mutant follicle cells are not markedly different in size from their heterozygous neighbours (O), supporting the idea that the Akt1¹ allele is not a null and can be suppressed by wdb. Egg chambers are at stage 10. Scale bars: 50 µm (E,U), 25 µm (A,I,M,Q,Y).