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Figure 1


Fig. 1. The behavior of wild-type mDia1 in XTC cells. (A) Single-molecule imaging of EGFP-mDia1Full speckles reveals different types of motility. Red lines show trajectories of mDia1Full speckles observed within a 6 second time window. Blue dotted lines indicate the contour of the cell edge (left). Representative trajectories of random (middle) and processive (right) mDia1Full speckles are shown. (B) mDia1Full speckles found within a 6 second time window are classified into the indicated groups (n=11 cells) as described within the text. (C) Motility of mDia1Full speckles in the random group is independent of actin polymerization. Random speckles were still observed 1 minute after 1 µM CytD or 1 µM LatB treatment. Trajectories of mDia1Full speckles found within a 6 second time window are shown.