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Figure 2


Fig. 2. Epsin-null mutants display defects in cytokinesis and spore morphology. (A) An immunoblot of whole cell lysates of the wild type (WT) and epsin-null mutants (epsin-) stained with anti-epsin antibodies. (B) Wild type (WT) and epsin mutants (epsin-) develop into fruiting bodies. Scale bar: 0.2 mm. (C) DIC images of spores harvested from fruiting bodies of wild-type cells (WT), epsin-null mutants (epsin-), and epsin-null mutants expressing epsin:GFP (epsin- +epsin:GFP). Expression of epsin:GFP restores wild-type sore morphology. Scale bar: 5 µm. (D) Wild type (WT), epsin-null mutants (epsin-), clathrin-null mutants (clathrin-), and epsin-null mutants expressing epsin:GFP (epsin- +epsin:GFP) grown in suspension for 72 hours and stained with DAPI to visualize nuclei. Scale bar, 5 µm. (E) Ratio of spore width:length in wild type (WT), epsin-null mutants (epsin-) and epsin-null mutants expressing epsin:GFP (epsin- +epsinGFP), n=50 for each cell line. (F) Quantification of multinucleated cells in suspension cultures of wild type (WT), epsin mutants (epsin-), epsin mutants expressing epsin:GFP (epsin- +epsin:GFP) and CHC mutants (clathrin-); n=300 for each cell line. Error bars are s.e.m.