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Figure 1


Fig. 1. PKC{epsilon}-/- mice show significantly reduced wound closure and presence of {alpha}-SMA-expressing myofibroblasts. (A) A 4 mm dermal punch was placed in the back of PKC{epsilon}+/+ and PKC{epsilon}-/-, and wound closure was measured. A total of six animals were analyzed (four wounds/animal). Eight-week-old females were assessed (*P<0.05, closure in PKC{epsilon}-/- significantly different from PKC{epsilon}+/+ mice). (B) Anti-{alpha}-SMA ({alpha}-SMA) staining of 14 day wounds. Sectioning and staining revealed the reduced presence of {alpha}-SMA-positive myofibroblasts (arrow). Sections probed with the {alpha}-SMA antibody were counterstained with Hematoxylin. Scale bar: 100 µm. (C) Western blot analysis of lysates prepared from of PKC{epsilon}+/+ and PKC{epsilon}+/+ wounds (25 µg/lane). Densitometry represents mean±s.d. of eight independent experiments (i.e. extracts from eight separate wounds; *P<0.05).