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Figure 1


Fig. 1. Association of endogenous nesprin-3{alpha}, plectin and vimentin with torsinA. Lysates from wild-type MFs were immunoprecipitated at 4°C overnight with antibodies to torsinA (D-M2A8 and DMG-10, 1:1) or IgG (anti-mouse IgG) without added ATP. Immunoprecipitates and lysates were resolved on SDS-PAGE gels and immunoblotted with antibodies to plectin, nesprin-3, vimentin, torsinA and GAPDH. TL, total lysates. The position of MW markers is indicated. The experiment was repeated three times and representative blots are shown. The percentage of each protein immunoprecipitated from lysates is: 7±2% for plectin, 17±4% for nesprin-3{alpha}, 17±3% for vimentin,12±3% for torsinA (values are mean percentage ± s.e.m., n=3 experiments).