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Figure 6


Fig. 6. Differential distribution of nesprin-3 in torsinA+/+ and torsinA-/- MEFs. (A) Double immunolabeling for the ER marker PDI and nesprin-3{alpha} in torsinA+/+ and torsinA-/- MEFs. (B) As A, but at lower magnification. (C) The relative proportion of immunoreactive nesprin-3{alpha} that was co-localized with the ER marker PDI in torsinA+/+ and torsinA-/- cells was assessed with a confocal microscope using the LSM 5 Pascal program, evaluating the co-localization coefficient for 150 cells of each cell type. (D) The relative number of co-localizing pixels in the red and green channels was evaluated in a value range 0-1 (0, no co-localization; 1, all pixels co-localize) and a significant difference was found between torsinA+/+ and torsinA-/- cells (n=3, P<0.0001, ANOVA). Error bars indicate s.e.m. Scale bars: 10 µm in A; 100 µm in B.