Fig. 4. Effect of TGF1 on expression of VEGF_xxxb and VEGF_total in podocytes. (A) ELISA of podocyte cell lysate for VEGF_xxxb and VEGF_total treated with TGFβ1. ^*P<0.05, ^**P<0.01 vs respective control, one-way ANOVA, Dunnett's test, n=5. (B) Lysates of podocytes treated with TGF1 resolved by western blot using antibodies that detect VEGF_xxxb. Densitometry of western blots show VEGF₁₆₅b to be upregulated relative to control (n=3). (C) RT-PCR of cells exposed to 1 nM TGFβ1. The lower band, corresponding to VEGF₁₆₅b is increased in intensity relative to the upper band (VEGF₁₆₅). Densitometry (n=5) indicates a significant upregulation of VEGF₁₆₅b relative to VEGF₁₆₅ (^**P<0.01, paired t-test).