Fig. 4. Single-stranded telomeric DNA accumulates in tgs1
cells. Genomic DNA was prepared in triplicate from wild-type and tgs1
cells, digested with XhoI and analyzed by non-denaturing (A) and denaturing (B) Southern blot analysis. WTd, DNA boiled for 10 minutes after digestion; positive control for single-stranded DNA hybridization.