Fig. 1. Kazrin protein levels increase during Ca²⁺-induced accumulation of terminally differentiating cells. (A) Immunoblotting of lysates from keratinocytes plated in the absence of feeders in low Ca²⁺ medium and then incubated in high Ca²⁺ medium for 0, 24, 48 or 72 hours. Band intensities were quantified and kazrin levels relative to t=0 normalized to actin are indicated below immunoblots. (B-E) Immunostaining for kazrin (green) and desmoplakin (red) in keratinocytes that were incubated in high Ca²⁺ medium for 6 (B,C) or 24 (D,E) hours. Nuclei were detected with DAPI counterstain (blue). Bars, 25 µm.