JCS -- Bikkavilli et al. 121 (21): 3598 Figure IG2

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Fig. 2. Suppression of G ${alpha}$ _q and G ${alpha}$ _s, but not G ${alpha}$ _o or G ${alpha}$ ₁₁, attenuates Wnt3a-induced p38 MAPK activation. F9 cells expressing Rfz1 were treated with siRNAs specific for G ${alpha}$ _o, G ${alpha}$ _q, G ${alpha}$ ₁₁ or G ${alpha}$ _s for 48 hours before treatment with Wnt3a for 15 minutes. p38 MAPK activity was then assayed. Upper panel displays mean values ± s.e.m. obtained from densitometer scanning of images from three independent experiments; the lower panel displays representative blots probed with anti-ATF2-P (p-ATF2), anti-p38 (p38), anti-G ${alpha}$ _o(G ${alpha}$ _o), anti-G ${alpha}$ _q (G ${alpha}$ _q), anti-G ${alpha}$ ₁₁(G ${alpha}$ ₁₁) and anti-G ${alpha}$ _s (G ${alpha}$ _s). ^*P<0.05 versus –Wnt3a control; ^#P<0.05 versus +Wnt3a control. The extent of knockdown of the G-protein ${alpha}$ -subunit expression was routinely 75% or more.