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Figure 4


Fig. 4. Bi-directional phase shifts induced by the REV-ERB{alpha} ligand on Bmal1::Luc Rat-1 cells or PER2::Luc lung slices. Individual cultures of confluent Rat-1 cells stably transfected with Bmal1::Luc reporter or ectopic lung slices from PER2::Luc mice were synchronized and subject to PMT recording. Cells or tissue slices were then treated with the compound (10 µM, red) or DMSO (black) at the indicated circadian time. Representative phase changes (horizontal arrow) after compound treatment (indicated by a vertical arrow) are shown in A and B (for Bmal1::Luc Rat-1), and D and E (for PER2::Luc lung slices). (C,F) Phase changes following the compound treatment are shown as mean ± s.e.m. n=3 for Bmal1::Luc Rat-1 cells, n=5 for the lung slices.