Fig. 3. The gp135-positive compact structure has hallmarks of apical recycling endosomes. Two individual fluorescence channels and the merged image represent maximum intensity projections of only confocal slices in which the compact structure was present. In the fourth column maximum intensity projections of merged fluorescence channels of the entire aggregate are overlaid with corresponding iso-colocalization surfaces (whitish objects) between gp135 (green) and indicated proteins (red) in aggregates with compact structures. The corresponding Pearson's correlation coefficient is shown on the right. Scale bars: 5 µm