Fig. 9. uPAR ligation results in dissociation of EGFR from caveolin and increased association of EGFR with phospho-caveolin. Fibroblast monolayers were incubated with 50 µM of either P25 or S25 for 1 hour. Cells were lysed in RIPA buffer. EGFR was immunoprecipitated from cell lysates using a mouse anti-EGFR antibody (3 µg) and the resulting immunoprecipitates were solubilized in gel buffer and analyzed by western blot for EGFR and (A) caveolin or (B) phospho-caveolin.