Fig. 6. The flagellar pocket protein CRAM is mislocalised in IFT140^RNAi cells. (A-C) Cells from the indicated cell lines were fixed and permeabilised with Nonidet before immunofluorescence with the anti-CRAM antiserum and stained with DAPI. Bottom panels show anti-CRAM signal only (white) and top panels show merged phase contrast, anti-CRAM (green) and DAPI (blue) images. (D-G) Cells from non-induced (D) and induced (E-G) IFT140^RNAi cells were fixed and incubated with the anti-CRAM antiserum without permeabilisation, followed by incubation with Protein AG coupled to gold particles. Arrows indicate short dilated flagella typical of retrograde transport inhibition; arrowheads indicate flagellar sleeves. Scale bars: 1 µm.