Fig. 7. Distribution of cytoskeletal and vimentin-associated proteins. (A,B) Immunofluorescence analysis of lens cryosections of (A) wt and (B) VimR₁₁₃C mice. Sections were stained using phalloidin to visualise F-actin. In mutant lenses actin localisation was not altered. (C,D) Double labelling of lens sections showing plectin (red) and vimentin (green). In control sections, both proteins were distributed underneath the plasma membrane. In VimR₁₁₃C lens sections, the submembraneous localisation of plectin was retained, whereas vimentin formed extensive aggregates. (E,F) Double immunofluorescence showing synemin (red) and vimentin (green). Synemin, which can heteropolymerise with vimentin, was relocalised to some but not all vimentin aggregates, suggesting that the interaction of vimentin and synemin is maintained, whereas their interaction to membrane attachment complexes is severed. Scale bars: 10 µm (A,B), 20 µm (C-F).