Fig. 7. H249A-D262A mutations on uPAR inhibit orthotopic lung tumor growth in vivo. (A) Tumor area is reduced in HD-mutant group and uPAR-knockdown group. WT or HD uPAR expressing H1299 cells or control (ctl) or uPAR knockdown (shu) H1299 cells were injected into the left lungs of nude mice (2x10⁵ in 20 µl of 10% Matrigel/mouse). 35 days later the lungs were harvested, embedded in OCT and serial sections were inspected for tumor size by examining green fluorescence. Average tumor area was measured using SimplePCI software and plotted: WT (n=20) and HD (n=22), Mann-Whitney U-test, **P<0.044; ctl (n=13) and uPAR knockdown (shu; n=14), Mann-Whitney U-test, *P<0.019. (B) Representative images of GFP-positive tumors. A view showing only a small part of the tumor mass from a lung injected with control cells and a view showing the whole tumor from injected uPAR-knockdown cells. The images were taken under x10 magnification by fluorescence microscopy using a Spot Camera and imaging software.