Fig. 5. Exclusion of membrane proteins from bulk endocytosis. Growth cones (2 DIV) incubated in KRH in the presence of FM4-64 (red) for 1 minute, fixed and retrospectively stained with antibodies against integral membrane proteins that are known to undergo recycling in axons (Trk, L1, β1 integrin, APP; green). The overlay between the corresponding fluorescence and DIC images is shown in the right panels. Scale bar: 10 µm.