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Figure 3


Fig. 3. Effect of mutations in RRM-1. (A) Positions of the F147L/F149L and {Delta}RRM-1 mutations. (B) Nuclear-cytoplasmic distribution of the mutants as determined by western blotting. Detection of p84 and tubulin was carried out to check for nuclear (p84) or cytoplasmic (tubulin) contamination of the two fractions. (C) Localization of both mutants detected by immunofluorescence microscopy in U2OS cells using anti-FLAG monoclonal antibody. The nuclear membrane was visualized using anti-LAP2β polyclonal antibody. (D) Typical nuclei of transfected cells are shown in detail, similar results were seen in HeLa cells. Scale bars: 10 µm.