Fig. 4. Chromatin fractionation of cells expressing endogenous and wt TDP-43, RRM-1 and F147L/F149L. Nuclei from transfected cells were digested with micrococcal nuclease and separated into fractions S1, S2 (supernatant) and P (pellet). The distribution of endogenous and wt TDP-43, F147L/F149L and RRM1 TDP-43 into the different nuclear fractions was detected by western blotting using antibodies against endogenous TDP-43 and FLAG-tagged transfected proteins. Histone H1 detection was used as control for the different nuclear fractions.