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Figure 5


Fig. 5. Biochemical fractionation of the C-terminally truncated mutants of TDP-43. (A) Diagram of the different mutants 1-366, 1-315 and {Delta}C. (B) Nuclear-cytoplasmic fractionation of HeLa cells expressing the different C-terminus mutants as detected by immunoblotting. Detection of p84 and tubulin was carried out to check for nuclear (p84) or cytoplasmic (tubulin) contamination of the two fractions.