Fig. 6. Number of MyHC-expressing cells per field. C2C12 cells were induced to differentiate after the indicated treatments, then harvested and fixed in graded methanol. Fixed cells were immunostained for MyHC expression. The number of nuclei per MyHC-positive cell (A) and the number of MyHC-positive cells per microscopical field (B) were scored. The histograms show the mean ± s.d. of three independent experiments. *P=0.005.