Fig. 7. Mitochondrial changes in apoptotic but not differentiating cells. C2C12 cells were treated with staurosporine (+ve) to induce apoptosis or were induced to differentiate. Differentiating cultures were washed to remove non-adherent cells. Cells were then harvested, lysed and centrifuged to obtain cytosolic and mitochondrial subcellular fractions. (A) The presence of mitochondrial proteins in the cytosolic fractions was then detected by immunoblotting. (B) Mitochondrial contamination of subcellular fractions was assessed by immunoblotting for COX IV. The results shown are typical of three independent experiments. (C,D) Alternatively, cells were harvested and stained with TMRE to detect m by flow cytometry. The results shown are typical of three independent experiments.