Fig. 7. Kif3B knockdown results in peripheral accumulation of TfR. (A-C) Kif3B siRNA1-treated cells incubated for 30 minutes with Tf-Alexa488 (A,C, green). Cells were then fixed and stained with anti-EEA1 antibodies (B,C, red). Asterisks in inset in C indicate the early endosomes containing Tf. Yellow in C represents the overlap between Tf-Alexa488 and EEA1. Scale bar: 5 µm.